use strict;
my $str = 'Chr1 74226650 . T C 50 . DP=385;VDB=0;SGB=-0.693147;RPB=0.982669;MQB=1;BQB=0.947576;MQ0F=0;AC=2;AN=2;DP4=0,95,0,289;MQ=20, GT:PL:DP, 1/1:78,127,0:384';
my $regex = qr/^chr(?:0?[1-9]|[1-9][0-9]|[MXY])\t0*[1-9][0-9]*\t[^\t]*(?:\t[ATCG]){2}\t/mip;
if ( $str =~ /$regex/g ) {
print "Whole match is ${^MATCH} and its start/end positions can be obtained via \$-[0] and \$+[0]\n";
# print "Capture Group 1 is $1 and its start/end positions can be obtained via \$-[1] and \$+[1]\n";
# print "Capture Group 2 is $2 ... and so on\n";
}
# ${^POSTMATCH} and ${^PREMATCH} are also available with the use of '/p'
# Named capture groups can be called via $+{name}
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for Perl, please visit: http://perldoc.perl.org/perlre.html