$re = '/^(\S+).*?(Transmembrane)\t(\w*)\t(\w*)/m';
$str = 'O75581 UniProtKB Transmembrane 1371 1393 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255
O75581 UniProtKB Topological domain 1394 1613 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255
O75581 UniProtKB Repeat 63 106 . . . Note=LDL-receptor class B 1
P13688 UniProtKB Transmembrane 429 452 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255
P13688 UniProtKB Topological domain 453 526 . . . Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255
P13688 UniProtKB Domain 35 142 . . . Note=Ig-like V-type;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:P31997
P19022 UniProtKB Transmembrane 725 745 . . . Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255
';
preg_match_all($re, $str, $matches, PREG_SET_ORDER, 0);
// Print the entire match result
var_dump($matches);
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for PHP, please visit: http://php.net/manual/en/ref.pcre.php