const regex = /NF.*?(\d*)\|.*/g;
// Alternative syntax using RegExp constructor
// const regex = new RegExp('NF.*?(\\d*)\\|.*', 'g')
const str = `NF3-9-16S_S9_1456|M02255:210:000000000-B9K3P:1:1101:14397:18152 1:N:0:9|o:50|m/o:0.720000|MR:n=0;r1=34;r2=2|Q30:p=97;p=100|CO:0|mismatches:36
NF3-9-16S_S9_9999|M02255:210:000000000-B9K3P:1:1101:14397:18152 1:N:0:9|o:50|m/o:0.720000|MR:n=0;r1=34;r2=2|Q30:p=97;p=100|CO:0|mismatches:36`;
const subst = `Re1_91910_$1`;
// The substituted value will be contained in the result variable
const result = str.replace(regex, subst);
console.log('Substitution result: ', result);
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for JavaScript, please visit: https://developer.mozilla.org/en/docs/Web/JavaScript/Guide/Regular_Expressions