re = /(?!(.).*\1)(?:[A2345]{5}|[23456]{5}|[34567]{5}|[45678]{5}|[56789]{5}|[6789T]{5}|[789TJ]{5}|[89TJQ]{5}|[9TJQK]{5}|[TJQKA]{5})/m
str = 'A2345
23456
34567
45678
56789
6789T
789TJ
89TJQ
9TJQK
TJQKA
52634
JQ89T
JQ89J'
subst = ''
result = str.gsub(re, subst)
# Print the result of the substitution
puts result
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for Ruby, please visit: http://ruby-doc.org/core-2.2.0/Regexp.html