$re = '/^define\(\s*[\'"]*(.*?)[\'"]*[\s,]+[\'"]*(.*?)[\'"]*\s*\)/mi';
$str = 'define("My_KEY", "My_Value l");
define(\'My_KEY\', \'My_Value\');
define( \'My_KEY\' , "My_Value" );
define( \'My_KEY\' , \'My\\\'_\\\'Value\' );
define( \'My_KEY\' , "My\'_\'Value" );';
preg_match_all($re, $str, $matches, PREG_SET_ORDER, 0);
// Print the entire match result
var_dump($matches);
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for PHP, please visit: http://php.net/manual/en/ref.pcre.php