use strict;
my $str = '(\'1234\', \'1234\', 1234, \'1234\'),
(\'1234\', \'1234\', 12, \'hello\'),
(\'stuff\', \'1234\', 1234, \'1234\'),
(\'1234\', \'1234\', 1234, \'1234\'),
(\'1234\', \'1234\', 12, \'hello\'),
(\'stuff\', \'1234\', 1234, \'1234\'),
(\'1234\', \'1234\', 1234, \'1234\'),
(\'1234\', \'1234\', 12, \'hello\'),
(\'stuff\', \'1234\', 1234, \'1234\'),
(\'1234\', \'1234\', 1234, \'1234\'),
(\'1234\', \'1234\', 12, \'hello\'),
(\'stuff\', \'1234\', 1234, \'1234\'),
(\'1234\', \'1234\', 1234, \'1234\'),
(\'1234\', \'1234\', 12, \'hello\'),
(\'stuff\', \'1234\', 1234, \'1234\'),
(\'1234\', \'1234\', 1234, \'1234\'),
(\'1234\', \'1234\', 12, \'hello\'),
(\'stuff\', \'1234\', 1234, \'1234\'),';
my $regex = qr/(?:^.*\r?\n){4}.*\K(,)$/mp;
my $subst = '';
my $result = $str =~ s/$regex/$subst/rg;
print "The result of the substitution is' $result\n";
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for Perl, please visit: http://perldoc.perl.org/perlre.html