$re = '/(?<=_)[^._]*(?=\.)/';
$str = 'India_AP_Dev1.txt
USA_GA_QA2.txt
USA_NY_AWSDev1.txt
AUS_AA_BB_QA4.txt';
preg_match_all($re, $str, $matches, PREG_SET_ORDER, 0);
// Print the entire match result
var_dump($matches);
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for PHP, please visit: http://php.net/manual/en/ref.pcre.php