$re = '/^(-\s+.*?)^([^-])/ms';
$str = '24
00:03:01,848 --> 00:03:04,893
- How adorable.
[both laughing]
48
00:02:53,798 --> 00:02:54,758
[clears throat]
49
00:02:57,552 --> 00:02:59,971
- [clears throat] Phil.
What can I get you?';
$subst = "$1- $2";
$result = preg_replace($re, $subst, $str);
echo "The result of the substitution is ".$result;
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for PHP, please visit: http://php.net/manual/en/ref.pcre.php