$re = '/^(?=[^@]*[a-z])(?![^@\r\n]*(?:\+\.|\.\+))[a-z\d+_.-]+@[a-z\d-]+\.[a-z\d-]*[a-z]$/mi';
$str = 'a1+-_.@2b-.c3-d
_1+-_.@2b-.c3-d
a1$-._@2b-.c3-d
a1+.-2@2b-.c3-d
a1.+_.@2b-.c3-d
a1+-_.@2$-.c3-d
a1+-_.@2.b.c3-d
a1+-_.@2b-.$3-d
a1+-_.@2b-.c3-4
';
preg_match_all($re, $str, $matches, PREG_SET_ORDER, 0);
// Print the entire match result
var_dump($matches);
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for PHP, please visit: http://php.net/manual/en/ref.pcre.php