use strict;
my $str = '00:21:06,200
00:2106,200 or if 00:21x06,200
00:12345,67';
my $regex = qr~\b\d\d:\K(\d\d)[x:\d]?(?=\d\d\b)~mp;
my $subst = '$1:';
my $result = $str =~ s~$regex~$subst~rg;
print "The result of the substitution is' $result\n";
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for Perl, please visit: http://perldoc.perl.org/perlre.html