# If you'd like to omit non-matching lines from the result; add ';d' to the end of the expression.
sed -E 's/^(\s*(?<!#)\s*PRIMER_PRODUCT_SIZE_RANGE\s*)=.*$/\1=500-300/gm;t' <<< "# PRIMER_PRODUCT_SIZE_RANGE=xxx-yyy
PRIMER_PRODUCT_SIZE_RANGE=100-300
PRIMER_NUM_RETURN=3
PRIMER_MAX_END_STABILITY=9.0
PRIMER_MAX_HAIRPIN_TH=24.0"
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for SED, please visit: https://www.gnu.org/software/sed/manual/html_node/The-_0022s_0022-Command.html