$re = '@(http://)\S*(\.test\.mywebsite\.com:8080)@m';
$str = 'File 1 :
myVarString=abc
myVarUrl=http://aaaaa.test.mywebsite.com:8080
File 2 :
myVarString=abc
myVarUrl=http://bbbbbbbbbbb.test.mywebsite.com:8080
File n :
myVarString=abc
myVarUrl=http://nnnnnnnnnnn.test.mywebsite.com:8080
';
$subst = "$1some_new_value$2";
$result = preg_replace($re, $subst, $str);
echo "The result of the substitution is ".$result;
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for PHP, please visit: http://php.net/manual/en/ref.pcre.php