re = /^.*Your Username:.(.*)(?!.Your Password)$/
str = 'Subscription/Member ID: 1655107153 Your Username: jose1234
Your Password: jose1234'
subst = '$1'
result = str.gsub(re, subst)
# Print the result of the substitution
puts result
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for Ruby, please visit: http://ruby-doc.org/core-2.2.0/Regexp.html