use strict;
my $str = 'subject name="Biology" value="12th"
topic name="system" value="anything"
topic
subject name="mathematics" value="12th"
topic name="system" value="anything"
other topics ="anything" value="anything"
topic
subject name="chem102" value="12th"
topic name="system" value="anything"
topic
subject name="ComputerSc" value="12th"
topic name="system" value="anything"
other topics ="anything" value="anything"
topic';
my $regex = qr/(subject name=\"(?:Biology|chem102)\"[\s\S]+?)\r?\n^topic$|(^topic$\r?\n)/mp;
my $subst = '\\1\\2\\n';
my $result = $str =~ s/$regex/$subst/rg;
print "The result of the substitution is' $result\n";
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for Perl, please visit: http://perldoc.perl.org/perlre.html