use strict;
my $str = 'http://cfile21.uf.tistory.com/original/212BA64E563633880B731B
http://cfile21.uf.tistory.com/original/212BA64E563633880B731B
http://cfile21.uf.tistory.com/original/212BA64E563633880B731B';
my $regex = qr/(http:\/\/cfile([0-9]+)(.)([a-z]+)(.)tistory.com\/original\/([a-zA-z0-9]+))(?![\s\S]*\1)/p;
my $subst = '';
my $result = $str =~ s/$regex/$subst/rg;
print "The result of the substitution is' $result\n";
Please keep in mind that these code samples are automatically generated and are not guaranteed to work. If you find any syntax errors, feel free to submit a bug report. For a full regex reference for Perl, please visit: http://perldoc.perl.org/perlre.html